Protein Solubility Calculator
Combine measured dissolved protein mass with solvent volume to estimate solubility percentage and concentration.
Percent soluble
90 %
Soluble concentration
0.45 mg/mL
Undissolved mass
5 mg
How to Use This Calculator
Prepare suspension
Record the total protein mass added to the solvent volume of interest.
Measure dissolved fraction
After centrifugation or filtration, measure the protein remaining in solution.
Enter values above
Provide total mass, dissolved mass, and solvent volume to compute solubility metrics.
Compare formulations
Use the calculated percent and concentration to compare buffer conditions or additives that enhance solubility.
Formula
Percent soluble = (dissolved / total) * 100
Concentration = dissolved / volume
Ensure masses are in mg and volumes in mL to obtain mg/mL. Adjust units consistently for other measurement systems.
Example
Starting with 50 mg protein in 100 mL solvent, if 45 mg remains dissolved: percent soluble = 45/50 * 100 = 90 percent, concentration = 45/100 = 0.45 mg/mL.
Full Description
Protein solubility dictates formulation stability, bioprocess yields, and crystallization success. Quantifying soluble fractions helps optimize salt, pH, and additive screens.
This calculator provides a quick mass balance approach, assuming undissolved protein is removed completely during clarification steps.
Frequently Asked Questions
How do I measure dissolved protein?
Use absorbance assays, Bradford assays, or other quantitative protein methods on the clarified supernatant.
What if some protein forms aggregates?
Aggregated or precipitated protein counts as undissolved. Adjust buffers or additives to increase solubility.
Can I change units?
Yes. Use grams and liters for larger batches. Concentration units follow the mass and volume inputs you provide.
Does temperature matter?
Protein solubility often depends on temperature. Record the assay temperature when comparing results.
Should I correct for dilution?
Include any additional solvent added during clarification when calculating the final solvent volume.