Resuspension Calculator
Quickly compute how much solvent is needed to reach a desired concentration or what concentration results from a fixed volume.
Leave blank to compute required volume. Enter to compute achievable concentration.
Required solvent volume
5 mL
How to Use This Calculator
Weigh lyophilized reagent
Record the total dry mass that will be resuspended.
Set target concentration
Enter the desired final concentration in mg/mL or other consistent units.
Optionally fix final volume
Provide the final volume if predetermined to compute the resulting concentration instead.
Resuspend with calculated volume
Add the calculated solvent volume (or adjust concentration as informed by the calculator).
Formula
V = m / C
C = m / V
m is mass, C target concentration, V solvent volume. Units must be consistent (for example mg and mL).
Example
To prepare 1 mg/mL from 5 mg protein: V = 5 / 1 = 5 mL solvent. If only 2 mL is available, concentration becomes 5 / 2 = 2.5 mg/mL.
Full Description
Resuspension calculations help prepare stock solutions accurately, avoiding overly concentrated or dilute preparations when reconstituting lyophilized reagents.
Simple mass balance enables quick conversions between mass, volume, and concentration without manual unit juggling.
Frequently Asked Questions
Can I enter grams instead of milligrams?
Yes. Use consistent units so that the resulting concentration units match your inputs.
What about volume losses?
Account for pipette dead volume or adsorption by adding a small excess above the calculated volume.
How do I adjust for stock dilution?
Apply dilution factors separately after preparing the stock according to the calculator output.
Can the reagent be insoluble?
If undissolved material remains, report actual dissolved mass rather than total mass to determine the effective concentration.
Does temperature matter?
Solubility can be temperature dependent. Dissolve at the recommended temperature for the reagent.