Semen Analysis Reference Tool
Enter laboratory results to compare with WHO 5th percentile reference values. Use this tool to guide repeat testing and specialist referrals.
WHO lower reference: 1.4 mL
WHO lower reference: 16 million/mL
WHO lower reference: 42%
WHO lower reference: 30%
WHO lower reference: 4%
Typical range: 7.2 - 8.0
Total sperm count
40.0 million
Progressive motile count
12.8 million
Parameter Summary
Volume
2.00 mL
Reference: >=1.4 mL
Volume within WHO 2021 reference range.
Concentration
20.0 million/mL
Reference: >=16 million/mL
Concentration meets WHO reference value.
Total motility
50.0%
Reference: >=42%
Total motility within reference limits.
Progressive motility
32.0%
Reference: >=30%
Progressive motility adequate for natural conception.
Morphology (normal forms)
5.0%
Reference: >=4%
Morphology meets WHO reference range.
pH
7.4
Reference: 7.2 - 8.0
pH within expected range.
How to Use This Calculator
Obtain a WHO-compliant semen analysis
Ensure 2-5 days abstinence, complete sample collection, and laboratory processing within 1 hour.
Enter the measured parameters
Provide volume, concentration, motility components, morphology, and pH for comparison with reference ranges.
Interpret results in clinical context
Abnormal findings warrant repeat testing, lifestyle review, and possible referral to a reproductive urologist.
Formula Reference
Total sperm count = Volume (mL) * Concentration (million/mL)
Progressive motile count = Total sperm count * Progressive motility (%) / 100
WHO 2021 lower reference limits: Volume ≥ 1.4 mL, Concentration ≥ 16 million/mL, Total motility ≥ 42%, Progressive ≥ 30%, Normal morphology ≥ 4%
Frequently Asked Questions
How many semen analyses are needed?
Obtain at least two samples 2-3 weeks apart. Semen quality fluctuates; use the average for diagnosis.
Can medication improve semen parameters?
Address underlying causes first (varicocele, hormonal issues). Antioxidants and lifestyle changes may help; evidence varies.
Does morphology alone predict IVF success?
No. Morphology should be interpreted alongside count and motility. Intracytoplasmic sperm injection (ICSI) bypasses most morphology issues.
What if the sample pH is low?
Acidic semen (<7.2) may indicate ejaculatory duct obstruction or seminal vesicle agenesis. Imaging and specialist referral are recommended.